ZVIRI KUSHANDISA
The StrongStep^®Adenovirus Rapid Test Device (Feces) inokurumidza kuonaimmunoassay yemhando yekufungidzira yeadenovirus mumunhufecal specimens.Iyi kit yakagadzirirwa kushandiswa seyamuro mukuongororwa kweadenovirus
utachiona.

NHUNGAMIDZO
Enteric adenoviruses, kunyanya Ad40 neAd41, ndiwo anonyanya kukonzera manyoka.muvana vakawanda vanorwara nechirwere chemanyoka chechipirichete kune rotavirus.Chirwere chemanyoka ndicho chikonzero chikuru chekufamuvana vadiki pasi rose, kunyanya munyika dzichiri kusimukira.Adenovirusutachiona hwakaparadzaniswa pasi rose, uye hunogona kukonzera manyokamuvana gore rose.Utachiona hunowanzoonekwa muvana vashoma panemakore maviri ezera, asi akawanikwa muvarwere veese mazera.Zvidzidzo zvinoratidza kuti adenoviruses inosanganiswa ne4-15% yevosemuchipatara zviitiko zveviral gastroenteritis.

Kukurumidza uye kwakarurama kuongororwa kweadenovirus-related gastroenteritis inobatsiramukumisikidza etiology ye gastroenteritis uye ine chekuita nemurwere manejimendi.Dzimwe nzira dzekuongorora dzakadai seelectron microscopy (EM) uyenucleic acid hybridization inodhura uye inobata basa.Given thekuzvidzora maitiro ehutachiona hweadenovirus, inodhura uyebvunzo dzebasa dzinogona kunge dzisiri madikanwa.

PRINCIPLE
Iyo Adenovirus Rapid Test Device (tsvina) inoona adenoviruskuburikidza nekuona kududzirwa kwemavara ekukura kwemukatistrip.Anti-adenovirus ma antibodies haafambise munzvimbo yekuyedza yemembrane.Panguva yekuedzwa, mufananidzo wacho unopindirana ne-anti-adenovirus antibodiesyakasanganiswa kune zvimedu zvine mavara uye yakafanoiswa pane yemuenzaniso pad yebvunzo.Musanganiswa wacho unobva watama nepakati pe membrane ne capillary action uye inopindiranaine reagents pa membrane.Kana pane adenovirus yakakwana mumuenzaniso, abhendi remavara richaita panzvimbo yekuyedza ye membrane.Kuvapo kweizvibhandi remavara rinoratidza chigumisiro chakanaka, asi kusavapo kwayo kunoratidza kuipamhedzisiro.Kuonekwa kwebhandi remavara panzvimbo yekutonga kunoshanda seprocedural control, zvichiratidza kuti vhoriyamu yakakodzera yemuenzaniso yaveyakawedzerwa uye membrane wicking yaitika.

PROCEDURE
Huya nemiedzo, zvienzaniso, buffer uye/kana zvidzoreso kune tembiricha yemumba(15-30 ° C) isati yashandiswa.
1. Kuunganidzwa kwemuenzaniso uye kusati kwarapwa:
1) Shandisa midziyo yakachena, yakaoma yekuunganidza sampuli.Zvibereko zvakanakisisa zvichaveyakawanikwa kana kuongororwa kunoitwa mukati meawa 6 mushure mekuunganidzwa.
2) Yezvienzaniso zvakasimba: Bvisa uye bvisa dilution tube applicator.Ivachenjerera kuti usateura kana kumwaya mushonga kubva muchubhu.Unganidza mienzanisonekuisa chimuti cheapplicator munzvimbo dzinenge 3 dzakasiyana dzetsvina yekuunganidza inenge 50 mg yetsvina (yakaenzana ne1/4 yepizi).Yezviyero zvemvura: Bata iyo pipette yakatwasuka, aspirate fecalmienzaniso, uye wozoendesa madonhwe maviri (anenge 80 µL) muspecimen yekuunganidza chubhu ine buffer yekubvisa.
3) Dzorera chibatiso muchubhu uye pukuta chivharo zvakasimba.Ivachenjerera kuti usatyore muromo wechubhu ye dilution.
4) Kuzunungusa iyo specimen yekuunganidza chubhu zvine simba kusanganisa muenzaniso uyeiyo buffer yekubvisa.Mienzaniso yakagadzirirwa mumuenzaniso wekuunganidza chubhuinogona kuchengetwa kwemwedzi mitanhatu pa -20 ° C kana isina kuongororwa mukati meawa imwe mushurekugadzirira.

2. Kuedza
1) Bvisa bvunzo kubva muhomwe yayo yakavharwa, woiisa pairinzvimbo yakachena, yakaenzana.Nyora bvunzo nemurwere kana kutongaidentification.Kuti zvive nemigumisiro yakanaka, kuongorora kunofanira kuitwa mukati meimweawa.
2) Uchishandisa bepa rematishu, pwanya muromo wechubhu ye dilution.Bataiyo chubhu yakatwasuka uye bvisa madonhwe matatu emushonga mumuenzaniso mutsime(S) yebvunzo mudziyo.Dzivisa kuteya mabhuru emhepo mumuenzaniso zvakanaka (S), uye usawedzera
chero mhinduro kuhwindo remhedzisiro.Sezvo bvunzo inotanga kushanda, ruvara rwunofamba nepakati pe membrane.

3. Mirira kuti bhendi (ma) mavara rionekwe.Mhedzisiro inofanira kuverengwa pa10maminitsi.Usadudzire mhinduro mushure memaminitsi makumi maviri.

Cherechedza:Kana iyo muenzaniso isingatame nekuda kwekuvapo kwezvikamu, centrifugeiwo akatorwa emhando dzirimo mune yekubvisa buffer vial.Unganidza 100 µL yemashura, buritsa mumuenzaniso mutsime (S) wechinhu chitsva chekuyedza uye tanga zvakare, uchitevera mirairo yatsanangurwa pamusoro.

Certifications