Yakatarisirwa kushandiswa
Iyo yakasimba^®Adedovirus Rapid Ongorora Mudziyo (FECES) iri kukurumidza kuonaimmangasy yeiyo Ququitative Yekutanga Kuwanikwa kweAdenovirus mumunhufecal fungidzira. Iyi kit inoitirwa kushandiswa seyubatsiro mukuongororwa kweAdenovirus
utachiona.

Nhanganyaya
Enteric Adenoviruses, kunyanya Ad40 uye Ad41, inzira inotungamira iyaMuVazhinji Vana Vanotambura Nechirwere Chinyorwa CalluthEAL, Chechipirichete kuRotaviruses. Chirwere cheLuute Colute Chirwere chikuru cherufuMujaya vadiki pasi rese, kunyanya munyika dzichiri kusimukira. Adenovirusvafambi vagara vari voga pasi rese, uye vanogona kukonzera manyokaMuvana gore rakatenderedza. Hutachiona hunonyanya kuonekwa muvana zvishoma paneMakore maviri ekuzvarwa, asi akawanikwa mune varwere emazera ese.Fundo dzinoratidza kuti adoeneviruses anosanganiswa ne4-15% yezvesemhosva dzemazipa ehutachiona hwehutachiona hwehutachiona.

Kurumidza uye Kuongororwa Kwakakodzera kweAdenvirus-kwakanangana neGastroenteritis kunobatsirapakugadzira maEtiology yeGastroenteritis uye ine hukama nemurwere maneja.Mamwe maitiro ekuongorora akadai se elekroncopy (em) uyeNucleic acid hybridization inodhura uye yakashanda-zvakanyanya. Wakapihwakuzvidzikisira chimiro cheAdenvirus utachiona, kudhura kwakadaro uyeMiedzo-yakawandisa miedzo inogona kunge isiri madikanwa.

Nheyo
Iyo Adenovirus Rapid FICKiD Chishandiso (FECE) Inoona AdenovirusKuburikidza nekududzira kwekuona kwekuvandudza kwemavara pane zvemukatistrip. Anti-adenovirus antibodies antibodies anosvibiswa pane iyo test nzvimbo yemembrane. Munguva yekuyedza, iyo fungidziro inosangana neAnti-adenovirus Antibodieskuunganidzwa kune zvimedu zvemavara uye zvakaringana mumuenzaniso wekuenzanisira webvunzo.Musanganiswa wobva watama kupfuura membrane neCapillary chiito uye inowiriranane reagents pane membrane. Kana paine zvakakwana Adenovirus mune iyo fungidziro, aband ine ruvara ruchaumbwa kunzvimbo yeyedzo yemibvunzo yeMembrane. Kuvapo kweizvibhandi reruvara rinoratidza mhedzisiro yakanaka, nepo kusavapo kwayo kunoratidza zvakaipamhedzisiro. Kutaridzika kwebhendi renzo reropa panzvimbo yekutonga inoshanda setsika yekudzora, zvichiratidza kuti iyo yakakwana vhoriyamu yefungidziro yaveYakawedzerwa uye membrane wick yaitika.

Maitiro
Kuunza bvunzo, kufungidzira, buffer uye / kana kudzora kumhepo tembiricha(15-30 ° C) usati washandisa.
1. Kufungidzira kuunganidzwa uye pre-kurapwa:
1) Shandisa yakachena, yakaoma midziyo yekufungidzira kuunganidzwa. Mhedzisiro yakanakisa ichaveyakawanikwa kana leaday ichiitwa mukati memaawa matanhatu mushure mekuunganidzwa.
2) yeiyo solidmindens: unscrew uye bvisa diliution tube tube chikumbiro. Bechenjera kuti urege kupaza kana spatter solution kubva muchubhu. Unganidza kufungidziranekuisa iyo yemushandi weti mumatanho matatu akasiyana eFECES kuunganidza dzinenge makumi mashanu emamg of fece (zvakaenzana ne1 /4 yepea).Yemvura inofungidzira: bata pipette yakatwasuka, isipirate fecalfungidziro, uye wobva wachinjisa 2 madonhwe (anenge 80 μL) mukatifungidziro yekuunganidza tube ine iyo yekubvisa buffer.
3) Tsiva iyo yekunyorera kumashure mune iyo tube uye screw the cap yakasimba. Bechenjera kuti urege kutyora chipenga cheiyo dilution tube.
4) zunza muyero wekuunganidza tube nesimba kusanganisa iyo fungidziro uyeiyo yekubvisa buffer. Fungidziro yakagadzirirwa mune yefungidziro yekuunganidza tubeinogona kuchengetwa kwemwedzi mitanhatu at -20 ° C kana isina kuedzwa mukati meawa imwe chete mushurekugadzirira.

2. Kuedza
1) Bvisa muedzo kubva muhomwe yakavanzika, uye uiisenzvimbo yakachena, yakajeka. Label bvunzo nemurwere kana kutongakuzivikanwa. Zvekubatsira zvakanakisa, leary inofanira kuitwa mukati meimweawa.
2) Uchishandisa chidimbu chepepa reTishu, pwanya iyo tip yeiyo dilution tube. BatisisaIyo tube yakatwasuka uye yekubvarura 3 madonhwe emhinduro mufungidziro tsime(S) yeiyo bvunzo mudziyo.Dzivisa kuteedzera mhepo mabhuru mune fungidziro tsime (s), uye usawedzera
chero mhinduro yepahwindo.Sezvo bvunzo inotanga kushanda, ruvara ruchatamira kuyambuka membrane.

3. Mirira bhendi reruvara (s) kuoneka. Mhedzisiro yacho inofanira kuverengerwa pagumimaminetsi. Usadudzira mhedzisiro mushure memaminitsi makumi maviri.

ONA:Kana iyo fungidziro ikasatama nekuda kwekuvapo kwezvidimbu, centrifugeiyo inobviswa mitezo iri mune yekubvisa buffer vial. Unganidza 100 μl yeSupernatant, kuparira mufungidziro zvakanaka (s) yeiyo itsva bvunzo mudziyo uye tanga zvakare, kutevera mirairo yakatsanangurwa pamusoro apa.

Certification