Ndeipi nzira yakanakisisa?
-Kuyedza kweSARS-CoV-2 Kutapukirwa Kwehutachiona
Panyaya dzakasimbiswa dzeCCIDID-19, dzakashuma zviratidzo zvekiriniki zvakajairika zvinosanganisira fivha, kukosora, myalgia kana kuneta.Zvakadaro zviratidzo izvi hazvisi zvakasiyana zveCCIDID-19 nekuti zviratidzo izvi zvakafanana nezvechimwe chirwere chine hutachiona senge furuwenza.Parizvino, hutachiona nucleic acid Real-Nguva PCR (rt-PCR), CT imaging uye mamwe hematology paramita ndiwo maturusi ekutanga ekuongororwa kwechirwere chetachiona.Mazhinji ma laboratory test kits akagadzirwa uye akashandiswa mukuyedza sampuli dzevarwere dzeCCIDID-19 neChinese CDC.1, US CDC2nemamwe makambani akazvimirira.IgG/IgM antibody bvunzo, serological test nzira, yakawedzerwawo senzira yekuongorora muChina yakagadziridzwa vhezheni yekuongorora uye nhungamiro yekurapa kweiyo novel coronavirus chirwere (COVID-19), iyo yakapihwa musi wa3 Kurume.1.Iyo hutachiona nucleic acid rt-PCR bvunzo ichiri ndiyo yazvino yakajairwa nzira yekuongorora yekuongororwa kweCOVID-19.
StrongStep®Novel Coronavlrus (SARS-COV-2)Multiplex Chaiyo-Nguva PCR Kit(kuonekwa kwemajini matatu)
Asi aya chaiwo-nguva PCR test kits, achitsvaga genetic zvinhu zvehutachiona, semuenzaniso mumhino, nemuromo, kana netsvina swabs, inotambura nezvakawanda zvisingakwanisi:
1) Iyi miedzo ine nguva refu yekuchinja uye yakaoma mukushanda;ivo kazhinji vanotora paavhareji kwemaawa maviri kusvika matatu kuburitsa mibairo.
2) Iyo PCR bvunzo inoda marabhoritari ane certified, midziyo inodhura uye nyanzvi dzakadzidziswa kushanda.
3) Kune dzimwe nhamba dzenhema dzisina kunaka dze rt-PCR yeCOVID-19.Zvinogona kukonzerwa nekudzikira kweSARS-CoV-2 hutachiona hwehutachiona hwepamusoro hwekufema swab specimen (Novel coronavirus inonyanya kutapurira pazasi pekufema turakiti, senge pulmonary alveoli) uye bvunzo haigone kuona vanhu vakapinda nehutachiona, vakapora, uye. akabvisa utachiona kubva mumiviri yavo.
Tsvagiridzo yakaitwa naLirong Zou et al4akawana kuti huwandu hwehutachiona hwepamusoro hwakaonekwa mushure mekutanga kwechiratidzo, nehuwandu hwehutachiwana hwakaonekwa mumhino kupfuura pahuro uye hutachiona nucleic acid yekudurura maitiro evarwere vane hutachiona hweSARS-CoV-2 yakafanana neyevarwere vane furuwenza.4uye inoratidzika zvakasiyana neiyo inoonekwa muvarwere vane hutachiona hweSARS-CoV-2.
Yang Pan et al5akaongorora serial samples (pahuro, sputum, weti, uye stool) kubva kuvarwere vaviri muBeijing ndokuona kuti hutachiona hwehuro uye sputum samples dzakakwira mukati memazuva 5-6 mushure mekutanga kwechiratidzo, sputum samples dzinowanzoratidza huwandu hwehutachiona kupfuura. huro swab samples.Hapana hutachiona RNA hwakaonekwa muweti kana stool samples kubva kuvarwere vaviri ava.
PCR bvunzo inongopa mhedzisiro kana hutachiona huchiripo.Iwo maedzo haakwanise kuona vanhu vakapinda nehutachiona, vakapora, uye vakabvisa hutachiona kubva mumiviri yavo.Muchimiro, vangangoita makumi matatu kusvika makumi mashanu muzana muzana vaive vakanakira PCR muvarwere vane chirwere checonavirus pneumonia.Varwere vazhinji vekoronavirus pneumonia havagone kuongororwa nekuda kweiyo negative nucleic acid bvunzo, saka havagone kuwana kurapwa kunoenderana nenguva.Kubva pane yekutanga kusvika kune yechitanhatu edition yegwaro, zvichitsamira chete pahwaro hwekuongororwa kweiyo nucleic acid bvunzo mhinduro, izvo zvakakonzera dambudziko guru kuvarapi. "Muridzo-muridzo" wekutanga, Dr. Li Wenliang, chiremba wemaziso kuWuhan Central. Hospital, akafa.Munguva yehupenyu hwake, akange aine matatu nucleic acid bvunzo panyaya yefivha uye kukosora, uye kekupedzisira paakawana PCR mhedzisiro.
Mushure mekurukurirano nenyanzvi, zvakasarudzwa kuwedzera nzira dzekuongorora serum senzira itsva yekuongorora.Nepo bvunzo dzeantibody, dzinonziwo serological bvunzo, dzinogona kusimbisa kana munhu akatapukirwa kunyangwe immune system yavo yabvisa hutachiona hunokonzera COVID-19.
StrongStep® SARS-COV-2 IgG/IgM Antibody Rapid Test
IgG/IgM antibody bvunzo ichabatsira kutsvaga nenzira yakawandisa-yakatarisana nevanhu vanga vaine hutachiona, nekuti nyaya zhinji dzinoita kunge dzakapararira kubva kuvarwere vasingaonekwi vasingazivikanwe zviri nyore.Vaviri muSingapore, murume akaonekwa aine PCR, mukadzi wake PCR bvunzo yainge isina, asi bvunzo dzeantibody dzakaratidza kuti aive nemasoja ekudzivirira chirwere, sezvakaita murume wake.
Serological assays inoda kunyatso simbiswa kuti ive nechokwadi chekuti vanoita nekuvimbika, asi chete kune masoja ekudzivirira chirwere anorwisa hutachiona.Chimwe chainetsa ndechekuti kufanana pakati pehutachiona hunokonzeresa acute acute kufema syndrome uye COVID-19 kunogona kutungamira mukuyambuka-reactivity.Iyo IgG-IgM yakagadzirwa naXue Feng wang6yaifungidzirwa kuti inogona kushandiswa seye-point-of-care test (POCT), sezvo inogona kuitwa pedyo nemubhedha neropa remunwe.Iyo kiti ine sensitivity ye88.66% uye chaiyo ye90.63%.Zvakadaro, pakanga pachine mhedzisiro yenhema uye yenhema yakaipa.
MuChina yakagadziridzwa vhezheni yekuongorora uye nhungamiro yekurapa kweiyo novel coronavirus chirwere (COVID-19)1, Mhosva dzakasimbiswa dzinotsanangurwa semhosva dzinofungidzirwa dzinosangana chero ipi yeiyi nzira dzinotevera:
(1) Sampuli dzeturakiti rekufema, sampuli dzeropa kana stool dzakaongororwa kuti dzine SARS-CoV-2 nucleic acid uchishandisa rt-PCR;
(2) Genetic kutevedzana kwehutachiona kubva mukufema turakiti, ropa kana stool samples samples ine homologous neiyo inozivikanwa SARS-CoV-2;
(3)Serum novel coronavirus chaiyo IgM antibody uye IgG antibody yaive yakanaka;
4
Kuongororwa uye kurapwa kweCOVID-19
| Guidelines | Pubished | Yakasimbiswa yekuongorora maitiro |
| Version 7th | 3Kurume 2020 | ❶ PCR ❷ NGS ❸ IgM+IgG |
| Version 6th | 18 Feb.2020 | ❶ PCR ❷ NGS |
Reference
1. Mazano ekuongororwa uye kurapwa kweiyo novel coronavirus pneumonia (yekuedza vhezheni yechinomwe, National Health Commission yePeople's Republic of China, yakapihwa na3. Mar.2020)
http://www.nhc.gov.cn/yzygj/s7652m/202003/a31191442e29474b98bfed5579d5af95.shtml
2. Tsvagiridzo Shandisa Chete-Chaiyo-Nguva RT-PCR Protocol yeKuzivikanwa kwe2019-nCoV.
https://www.cdc.gov/coronavirus/2019-ncov/lab/rt-pcr-detection-instructions.html
3. Singapore inoti kekutanga kushandisa antibody bvunzo kuronda hutachiona hwe coronavirus
https://www.sciencemag.org/news/2020/02/singapore-claims-first-use-antibody-test-track-coronavirus-infections
4.SARS-CoV-2 Viral Load muUpper Respiratory Secimens yeVarwere Vakatapukirwa Kukadzi 19,2020 DOI: 10.1056/NEJMc2001737
5.Viralloads yeSARS-CoV-2 mumakiriniki samples Lancet Infect Dis 2020 Yakabudiswa Pamhepo Kukadzi 24, 2020 (https://doi.org/10.1016/S1473-3099(20)30113-4)
6. Kuvandudza uye Kliniki Kushandiswa kweA Rapid IgM-IgG Yakasanganiswa Antibody Test yeSARS-CoV-2.
Utachiona Kuongororwa XueFeng Wang ORCID iD: 0000-0001-8854-275X
Nguva yekutumira: Mar-17-2020
