Procalcitonin Test
ZVIRI KUSHANDISA
The StrongStep®Procalcitonin Bvunzo inokurumidza immune-chromatographic assay yehafu-huwandu hwekuonekwa kweProcalcitonin muserum yemunhu kana plasma.Inoshandiswa pakuongorora nekudzora kurapa kwakanyanya, hutachiona hwehutachiona uye sepsis.
NHUNGAMIDZO
Procalcitonin (PCT) ipuroteni diki iyo inosanganisira 116 amino acid masara ane huremu hwemamorekuru hunosvika 13 kDa iyo yakatanga kutsanangurwa naMoullec et al.mu 1984. PCT inogadzirwa kazhinji muC-masero ematokisi.Muna 1993, chiyero chepamusoro chePCT muvarwere vane chirwere chetachiona chebhakitiriya yakabva yakashumwa uye PCT ikozvino inofungidzirwa kuti ndiyo inonyanya kukosha yezvipingamupinyi zvinosangana ne systemic inflammation uye sepsis.Kukosha kwekuongorora kwePCT kwakakosha nekuda kwehukama hwepedyo pakati pePCT kusungwa uye kuoma kwekuputika.Yakaratidzwa kuti "kuzvimba" PCT haina kugadzirwa muC-masero.Masero eeuroendocrine mavambo anogona kunge ari sosi yePCT panguva yekuzvimba.
PRINCIPLE
The StrongStep®Procalcitonin Rapid Test inoona Procalcitonin kuburikidza nekududzira kwekuona kwekukura kwemavara pane yemukati tambo.Procalcitonin monoclonal antibody haifambiswi panzvimbo yekuyedza ye membrane.Panguva yekuyedzwa, mufananidzo wacho unopindirana nemonoclonal anti-Procalcitonin masoja ekudzivirira chirwere akasanganiswa kune zvimedu zvine mavara uye akavharirwa pane conjugate pad yebvunzo.Musanganiswa unobva wafamba nepakati pe membrane ne capillary action uye unopindirana nema reagents pa membrane.Kana paine Procalcitonin yakakwana mumuenzaniso, bhendi reruvara richaumbwa munzvimbo yekuyedza ye membrane.Kuvapo kwebhandi iri remavara kunoratidza mhedzisiro yakanaka, nepo kusavapo kwayo kunoratidza mhedzisiro yakaipa.Kuonekwa kwebhandi remavara panzvimbo yekutonga inoshanda sekutonga kwemaitiro, zvichiratidza kuti chiyero chakakodzera chemuenzaniso chakawedzerwa uye membrane wicking yakaitika.Kuvandudzwa kweruvara kwakasiyana munharaunda yebvunzo (T) kunoratidza mhedzisiro yakanaka nepo huwandu hweProcalcitonin hunogona kuongororwa semi-huwandu nekuenzanisa kusimba kwemutsara webvunzo kune kusimba kwemutsara wereferenzi pane kadhi rekududzira.Kusavapo kwemutsara weruvara munharaunda yebvunzo (T)
inoratidza mhedzisiro yakaipa.
ZVEKUNGWARIRIRA
Iyi kit ndeye IN VITRO yekuongorora kushandiswa chete.
∎ Kiti iyi ndeyekungo shandisa PROFESSIONAL chete.
■ Verenga mirairo nemazvo usati waita bvunzo.
■ Chigadzirwa ichi hachina chero zvinhu zvinobva kuvanhu.
■ Usashandise zvinyorwa zvekiti mushure mezuva rekupera.
∎ Bata zvienzaniso zvese zvinogona kutapurira utachiona.
■ Tevedzera nzira yakajairwa yeLab uye nhungamiro yekuchengetedzwa kwezvipenyu pakubata nekurasa zvinhu zvingango konzera utachiona.Kana iyo assay maitiro apera, bvisa zvienzaniso mushure me autoclaving iwo pa121 ℃ kweinenge 20 min.Neimwe nzira, vanogona kurapwa ne 0.5% Sodium Hypochlorite kwemaawa vasati varaswa.
■ Usaite pipette reagent nemuromo uye kusaputa kana kudya paunenge uchiita maassays.
■ Pfeka magirovhosi panguva yese yekuita.