Kurudziro Yekukurumidza Kuedza
Yakatarisirwa kushandiswa
Iyo yakasimba®Kubvunzwa kwekuparidzwa ndeyezvinoonekwa zvishoma, Ququitative immunochromatographic quentation yeGEGBP-1 kubva kuAmniotic Fluid muVaginal Zvikamu zveVaginal panguva yekuzvitakura. Muedzo wacho unoitirwa nyanzvi yekushandisa kubatsira kuongororwa kuputika kwemitambo yemitambo (rom) muvakadzi vane pamuviri.
Nhanganyaya
Iko kusungwa kwe iGFBP-1 (insulin-senge kukura kwechimiro kusunga protein-1) muAmniotic Fluid ndeye 1000 nguva yakakwira kupfuura muSernal Serum. IGFBP-1 haisi kuzogara muVagina, asi mushure mekuputika kweFetal membranes, Amniotic Fluid neyekuwedzera kweGEGBP-1 inosanganisa zvigaro zveVaginal. Mune yakasimba ® prep ® yekubvunzwa, fungidziro yemavanda yemavanda inotorwa neyakagadzirwa polyester swab uye fungidziro inobviswa kuti isimbe yekufungidzira mhinduro. Kuvapo kweGEGBP-1 mumhinduro kunoonekwa uchishandisa kukurumidza bvunzo mudziyo.
Nheyo
Iyo yakasimba®Kubvunzwa kwekuviridzwa kunoshandisa mavara eurzivyaphi, Capillary kuyerera tekinoroji. Maitiro ekuedzwa anoda iyo solubilization ye iGfbp-1 kubva mubvu waginal swab nekusanganisa swab mune sampula buffer. Ipapo iyo yakasanganiswa sampula inowedzerwa kune iyo bvunzo cassette sampuli mushe uye musanganiswa anotama pamwe ne membrane nzvimbo. Kana IGFBP-1 iripo mumuenzaniso, ichaumba kuomarara neyekutanga ant- ugfbp-1 antibody conjugated kusvika kumavara mavara. Iyo yakaoma ichazosungwa neyechipiri ANTIGFBP-1 Anti munhu akavezwa pane iyo nitrocellulose membrane. Kuonekwa kweiyo yakaonekwa bvunzo mutsara pamwe nemutsara wekutonga ucharatidza mhedzisiro.
Kit zvikamu
| 20 Mumwe nemumwe packed test test | Chishandiso chimwe nechimwe chine mitsetse ine mavara ezvinyorwa uye reagents reagents pre-yakafukidzwa munzvimbo dzinoenderana. |
| 2KubviswaBuffer vial | 0.1 m phosphate buffered saline (PBS) uye 0.02% Sodium Azide. |
| 1 Kubatsira Kwakanaka SWAB (pane chikumbiro chete) | Iine IGFBP-1 uye Sodium Azide. Zvekutonga kwekunze. |
| 1 Kutadza Kwakaipa Swab (pane chikumbiro chete) | Kwete igfbp-1. Zvekutonga kwekunze. |
| 20 Zvidhiro | Zvekufungidzira kugadzirira kushandiswa. |
| 1 Workstation | Nzvimbo yekubata buffer vials uye machubhu. |
| 1 Package Insert | Yekuraira kwekushanda. |
Zvigadzirwa zvinodiwa asi zvisina kupihwa
| Timer | Zvekushandisa nguva. |
ZVEKUNGWARIRIRA
■ Kunyanzvi muVitro Diagnostic Shandisa chete.
■ Usashandise mushure mekupera zuva rakaratidzwa papakeji. Usashandise bvunzo kana foil yayo foil yakakuvadzwa. Usashandise mari yekushandisa.
■ Iyi kit ine zvigadzirwa zvemhuka. Kuziva kwakasimbiswa kweiyo mavambo uye / kana kuwanda kwemhuka hazvivimbise zvachose kusavapo kwekutumirwa pathogenic vamiririri. Naizvozvo, zvinokurudzirwa kuti zvigadzirwa izvi zviitwe sezvatinotapukira, uye vakabata kucherechedza nzvimbo dzekuchengetedza (usapinza kana kupinda kana inhale).
■ Regedza kuyambuka-kusvibiswa kwemufungidziro nekushandisa mutsva fungidziro yekuunganidza mudziyo kune yega yega fungidziro yakawanikwa.
■ Verenga maitiro ese nokungwarira usati waita chero bvunzo.
■ Usadye, inwa kana utsi munzvimbo inoonekwa nemifananidzo uye kits inobatwa. Bata zvese zvinongedzo sekunge zvine hutachiona hwevamiririri. Cherekedzai zvakatemwa pakudzivirira zvinopesana necrobiological njodzi muzvimiro zvese uye kutevera maitiro akajairwa ekuraswa kwakakodzera kwemufungidziro. Pfeka zvipfeko zvekudzivirira zvakadai semabhurosi emabhiza, magurovhosi ekudzivirira uye kuchengetedzwa kwemaziso kana fungidziro fungidziro.
■ Usataurirana kana kusanganisa reagents kubva kune dzakasiyana siyana. Usasanganisa Solution Bottle Caps.
■ Humidity uye tembiricha inogona kukanganisa zvine simba mhedzisiro.
■ Kana maitiro legey apedzwa, kurasa iyo swabs nokungwarira mushure mekuvabika navo pa121 ° C kwemaminetsi makumi maviri. Nenzira imwe cheteyo, ivo vanogona kurapwa ne 0.5% sodium hypochloride (kana imba-inobata bhururu) kweawa imwe isati yarwiswa. Zvishandiso zvinoshandiswa zvinoongororwa zvinofanirwa kuraswa zvinoenderana nenzvimbo, nyika uye / kana federal mitemo.
■ Usashandise cytology brushes nevarwere vane nhumbu.
Kuchengetera uye Kugadzikana
■ Kititi rinofanira kuchengetwa pa 2-30 ° C kusvikira iyo zuva rekupedzerwa rakadhindwa pamhenderekedzo yakasungwa.
■ Muedzo unofanira kuramba uri muchimbuzi wakasungwa kusvikira washandiswa.
■ Musasunungura.
■ Zvinotarisirwa zvinofanirwa kutorwa kuti udzivirire zvikamu mune ino kit kubva kusvibiswa. Usashandise kana paine humbowo hwekusvibiswa microbial kana kufungidzira. Kusvibiswa kwezvinhu zvidzimwe zvekupa zvigadzirwa, midziyo kana reagents zvinogona kutungamira mumhedzisiro yenhema.
Kuunganidza kuunganidza uye kuchengetedza
Shandisa chete Dacron kana rayon yakatwasirwa serile swabs ine mapurasitiki shafts. Iyo inokurudzira kushandisa iyo swab yakapihwa neKits mugadziri (iyo swabs haina miti iri, yeruzivo rwechiratidzo, ndapota bvunza mugadziri kana nhamba yemunharaunda, nhamba yecalage ndeye 207000). Swabs kubva kune vamwe vanopa vatengesi havana kusimbiswa. Swabs nemazano ekotoni kana masapiti emapuranga haakurudzirwe.
■ Muenzaniso unowanikwa uchishandisa polyester polyester swab. Muenzaniso unofanirwa kuunganidzwa usati waita bvunzo dzeDigital uye / kana transvaginal ultrasound. Chenjera kuti urege kubata chero chinhu neSwab usati watora sampuro. Nyatso isa chipenga cheiyo swab muBagina kune iyo posterior fornix kusvikira kurwisa kwasangana. Nenzira imwe cheteyo iyo sampu inogona kutorwa kubva kuPoseni Fornix panguva yekusimbisa kwepfuma. Iyo swab inofanira kusiiwa muVagina kwemasekondi gumi nemashanu kuti ibvumire kuti ibvise zvakavanzika zveVaginal. Dhonza iyo swab kunze nokungwarira !.
■ Isa iyo swab kune iyo yekubvisa tube, kana bvunzo inogona kumhanya nekukurumidza. Kana kuongororwa kwekukurumidza kusiri kuitika, samples dzemurwere dzinofanira kuiswa mune yakaoma yekufambisa tube yekuchengetedza kana kutakura. Iyo Swabs inogona kuchengetwa kwemaawa makumi maviri nemapfumbamwe pakupisa kwemakamuri (15-30 ° C) kana vhiki 1 pa 4 ° C kana kwete anopfuura matanhatu mwedzi pa -20 ° C. Zvese zviyero zvinofanirwa kubvumidzwa kusvika pakudziya kwemakamuri 15-30 ° C usati waedza.
Maitiro
Huya nemiedzo, fungidziro, buffer uye / kana kudzora kumhepo tembiricha (15-30 ° C) usati washandiswa.
■ Isa iyo yakachena yekubvisa tube munzvimbo yakatarwa yeiyo nzvimbo yekushanda. Wedzera 1ML yeChidimbu Buffer kune iyo yekubvisa tube.
■ Isa iyo fungidziro swab mune iyo tube. Sanganisa nesimba sanganisa mhinduro nekutenderera iyo swab ine simba kune iyo tube kwenguva kanomwe (uchinyoroveswa). Mhedzisiro yakanakisa inowanikwa kana iyo fungidziro yakasanganiswa nesimba mumhinduro.
E- Kanenge 1/2 yemuenzaniso wekuratidzira mhinduro inofanira kuramba iri muchubhu kune yakaringana capillary kufamba. Isa iyo cap mune inobvisa tube.
Rasa iyo swab mune yakakodzera biohacharard marara mudziyo.
■ Iyo nzira inobviswa inogona kuchengetedza tembiricha yemakamuri kwemaminetsi makumi matanhatu pasina kukanganisa mhedzisiro yebvunzo.
■ Bvisa muedzo kubva muhomwe yakavanzika, uye uiise pane yakachena, nzvimbo. Raira chishandiso chine murwere kana kudzora kuzivikanwa. Kuti uwane mhedzisiro yakanakisa, leaday inofanira kuitwa mukati meawa imwe.
■ Wedzera 3 madonhwe (anenge 100 μl) yeakabviswa sampuli kubva kuToxction tube kune sampuli zvakanaka pane bvunzo cassette.
Dzivisa kuteedzera mhepo mabhuru mune fungidziro zvakanaka (s), uye usakanda chero mhinduro muhwindo rekutarisa.
Sezvo bvunzo inotanga kushanda, uchaona ruvara runotenderera mimwe membrane.
■ Mirira bhendi renzou (s) kuoneka. Mhedzisiro yacho inofanira kuverengerwa pamaminitsi mashanu. Usadudzira mhedzisiro mushure memaminitsi mashanu.
Discard yakashandisa test tubes uye bvunzo cassette mune yakakodzera biohazarard marara emarara.
Nderpretation yeMhedzisiro
| ZvakanakaMhedzisiro:
| Mabhendi maviri emavara anoonekwa pane membrane. Rimwe bhendi rinoonekwa munzvimbo yekutonga (c) uye imwe bhendi inoonekwa munzvimbo yeyedzo (t). |
| Zvisina kunakaMhedzisiro:
| Bhendi rimwe chete reruvara chete rinoonekwa munzvimbo yekutonga (c). Hapana chinooneka band bhendi chinoonekwa munzvimbo yeyedzo (t). |
| ZvisingaitikeMhedzisiro:
| Control bhanji inotadza kuoneka. Mhedzisiro kubva kune chero bvunzo iyo isina kuburitsa bhendi rekutonga panguva yakatarwa yekuverenga inofanira kuraswa. Ndokumbirawo uongorore maitiro uye dzokorora nemubvunzo mutsva. Kana dambudziko rikaramba riripo, rikarega kushandisa kit ipapo uye bvunza wako kugoverwa. |
ONA:
1. Kukura kwerudzi munzvimbo yeTest (t) kunogona kusiyana zvichienderana nekuvharirwa kwezvinhu zvakaratirwa mune fungidziro. Asi zvinhu zvezvinhu hazvigone kutsanangurwa nemuyedzo uyu wekupedzisira.
2..
Mhando yekudzora
■ Yemukati yemamiriro emukati mashandiro anosanganisirwa muedzo. Bhendi reruvara rwemavara rinobuda munzvimbo yekutonga (c) inoonekwa seyakajeka yemukati maitiro ekudzora. Inosimbisa kukwana kwakakwana vhoriyamu uye kururamisa maitiro maitiro.
■ Expt Palater Runcedraces inogona kupiwa (pakukumbira chete) mune kits kuti ive nechokwadi chekuti bvunzo dziri kushanda nemazvo. Zvakare, kudzora kunogona kushandiswa kuratidza kuita kwakakodzera neyeye muyedzo wekubhadhara. Kuita bvunzo dzakanaka kana dzakaipa dzekutonga, zadzisa matanho ari muedzo procument chikamu ichibata iyo control swab nenzira yakafanana neyefungidziro swab.
Kugumira kwebvunzo
1. Hapana kududzirwa kwehuwandu kunofanirwa kuitwa zvichienderana nezvinoitika bvunzo.
2.Do usashandise bvunzo kana aluminamu yayo yaAlumini
3.A Yakasimba Yakasimba®Vakurudziro bvunzo mhedzisiro, kunyangwe ichiona kuvapo kweAnniotic fluid mumuenzaniso, haitsunge nzvimbo yekubvaruka.
4.Asine nemuedzo wese wekuongorora, mhedzisiro inofanira kududzirwa muchiedza chemamwe maclination.
5.Fif kubvarura yeFetal membranes kwakaitika asi kubhururuka kweAmbniotic Fluid yapera maawa gumi nemaviri isati yatorwa, inga gadziriso
Maitiro ekuita
Tafura: yakasimba®VHIKI TENDE VS. Imwe Brand Prim Bvunzo
|
Kusangana kwenyama: |
| Imwe mhando |
| ||
| + | - | Zvese | |||
| Yakasimba®Prom Bvunzo | + | 63 | 3 | 66 | |
| - | 2 | 138 | 140 | ||
|
| 65 | 141 | 206 | ||
Ongororo Inonakidza
Iyo yakaderera yekuona huwandu hweGEFBP-1 mune iyo inobviswa sampuli ndeyegumi nemairi gumi nemaviri:
Kupindira Zvinhu
Kuchengetwa kunofanirwa kutorwa kusasvibisa iyo yekunyorera kana Cervicicovaginal zvigaro zvine lubricants, soaps, disinfectants, kana kirimu. Lubriants kana kirimu inogona kukanganisa mumuviri nekunyura kweiyo fungidziro kune iyo anlicatator. SOaps kana disinfectants inogona kukanganisa neanti-antigen maitiro.
Zvingangoita zvinhu zvinopindira zvakaedzwa pakukatyiswa izvo zvinogona kuve zvinowanikwa zvine chekuita munzvimbo dzeCervicuginaginags. Zvinhu zvinotevera hazvina kupindira muley kana kuedzwa pamazinga anoratidza.
| Chinhu | Kusvinura | Chinhu | Kusvinura |
| Ampicillin | 1.47 MG / ML | Prostiglandin F2 | 0.033 MG / ML |
| Erythromycin | 0.272 MG / ML | Prostaglandin e2 | 0.033 MG / ML |
| Metnal urine 3rd trimester | 5% (vol) | Monistatr (Miconazole) | 0.5 mg / ml |
| Oxetocin | 10 IU / ML | Indigo carmine | 0.232 MG / ML |
| Terbutialine | 3.59 MG / ML | Gesamicin | 0.849 MG / ML |
| Dexamethasone | 2.50 mg / ml | Betadiner Gel | 10 mg / ml |
| MGSO4•7H2O | 1.49 MG / ML | Betadiner Cleanser | 10 mg / ml |
| Ritidrine | 0.33 MG / ML | K-YR jelly | 62.5 MG / ML |
| Dermicidolr 2000 | 25.73 MG / ML |
Zvinyorwa Zvitwo
Erisoglu and Mungan T. Kukosha Kwekuona Insulin - Kufanana nechero Chimiro Chekubatanidza - Kuenzanisa neNitrazine Test uye Amniotic Fluid vhoriyamu yekuongorora. Acta Obstet Gynecol SCand (2004) 83: 622-626.
Kubota t uye Takeuchi H. Kuongororwa kwe insulin-sekukura kwechimiro chinosunga protein - 1 seyakaongororwa chishandiso chekuparadza kwemambrames. J Orchet Gynecol Res (1998) 24: 411-417.
Rutanen em et al. Kuongororwa kwechikumbiro chechiedza cheInternu-seyakafanana nekukura chinhu chinosungira protein - 1 mukuongororwa kwemambres yemitambo. Clin chim actate (1996) 253: 91-101.
Rutanen em, Pekonen F, Karkkainen T. Kuyerwa kweInsulin-Sekureva Kwekuchinjisa Protein Impunasis In Humunasis Mukuongororwa Kwekupararira Kwemambrames. Clin chim actate (1993) 214: 73-81.
Glossary yezviratidzo
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| Catalog nhamba |  | Tembiricha yekuisa |
 | Bvunza mirayiridzo yekushandisa |
| Batch Code |
 | Mu vitro diagnostic chero chishandiso |  | Shandisa na |
 | Mugadziri |  | Ine zvakakwana |
 | Usashandise |  | Mumiriri Akatenderwa MuEuropean Community |
 | CE yakanyorwa zvichienderana neIVD Zvekushandisa Zvishandiso Zvepamhepo 98/79 / EC | ||
