Novel Coronavirus (Sars-Cov-2) Multiplex chaiyo-nguva PCR KT KRD
Izvi zvakanyanyisa kuterera, kugadzirira-kushandisa-shandisa pccr kt kombi inowanikwa mumhando yeLyophilized fomati (kusununguka-kuoma-kuoma maitiro) yekuchengetedza kwenguva refu. Iyo kit inogona kutakurwa uye yakachengetwa pamhepo tembiricha uye yakagadzikana kwegore rimwe. Imwe neimwe tube yePramux ine ese evagadziri anodiwa kune iyo PRC Izvo zvinongoda kuwedzera 13Ul yakatsemuka mvura uye 5ul inobviswa rna template, saka inogona kumhanya uye kukwidziridzwa pane iyo PRC zviridzwa.
Iyo QPCr muchina inofanira kuzadzisa zvinotevera zvinodiwa:
1. FIT 8 Strip PCR Tube vhoriyamu 0.2 ML
2. Iva nezvinopfuura manhamba mana ekucherechedzwa:
| Chiteshi | Kuenderana (NM) | Emission (NM) | Pre-calibrated dyes |
| 1. | 470 | 525 | Syb, Sybri Green i |
| 2 | 523 | 564 | Vic, Hex, Tet, Joe |
| 3. | 571 | 621 | Rox, Texas-Tsvuku |
| 4 | 630 | 670 | CY5 |
PRR-mapuratifomu:
7500real-time PCR system, Biorad cf96, ICYCLER IQ
Iko kuoma kwekutonhora keteni yekufambisa yeNovel Coronavirus nucleic acid yekuona Reagent
Kana nguva dzose nucle acid yekuona ndege inotakurwa muhurefu hurefu, iyo (-20 ± 5) Kuti uve nechokwadi chekuti tembiricha inosvika pachiyero, kilogiramu akati wandei echando chakaoma chinodiwa kune rimwe nerimwe bhokisi re nuclec acid kuongorora kusiri pasi pe50g, mazuva maviri kana matatu chete. Pane maonero ekuita kweindasitiri maitiro, uremu chaihwo hwevatariri vakapihwa nevanogadzira pane zvisingasviki 10% (kana zvishoma zvishoma pane kukosha uku) kwemudziyo. Yakawanda yehuremu inouya kubva kune yakaoma ice, ice mapaketi uye mabhokisi emabhokisi, saka mutengo wekufambisa wakanyanya kukwirira.
Muna Kurume 2020, kuwanda-19 kwakatanga kupaza kwakakura kune imwe nyika, uye kudiwa kweNovel Coronavirus nucleic acid yekuona zvinoshamisa. Kunyangwe yakanyanya mutengo wekuendesa kunze kwenzira dzevafundisi mune inotonhora keteni, vagadziri vazhinji vanogona vachiri kugamuchira nekuda kwehuwandu hukuru uye purofiti yakakwirira.
Nekudaro, nekuvandudzwa kwezvinhu zverudzi zvekutengesa zverudzi rwekunze kwezvirongwa zvekutengesa, pamwe nekusimudzirwa kweNational Control pamusoro peVanhu uye kusagadzikana munguva yekufambisa yeReagents, iyo yakakonzerwa nematambudziko ekutanga akakonzerwa nechekufambisa. Yakatambanudzwa nguva yekufambisa Izvi zvakanetseka zvakanyanya nucle acid reagents kutumira kunze kwenyika.
Tekinoroji yeLyofilillised yePCR Reagent yakabatsirwa kufambisa kweNovel Coronavirus nucleic lection reagent pasi rose
Iyo lyeppariled pcr Regents inogona kutakurwa uye inochengeterwa pamhepo tembiricha, iyo isingagoni kungoderedza mari yekufambisa, asi zvakare dzivirira matambudziko emhando yakakonzerwa nekufambisa. Naizvozvo, lyehlizing the reagent ndiyo nzira yakanakisa yekugadzirisa dambudziko rekutakura kunze kwekufambisa.
Lyotolization inosanganisira kusunungura mhinduro kuita nyika yakasimba, uyezve nekuzviisa pasi uye kupatsanura mupfusi wemvura pasi pecuum mamiriro. Kugadziriswa kwakaomeswa kuchiramba mumudziyo pamwe nekuumbwa kwakafanana uye chiitiko. Kuenzaniswa neakajairwa maredo reagents, iyo yakazara-chikamu lyephigiryi
Yakanyanya kusimba kupisa kusimba:Inogona nekumira kurapwa na56】 kwemazuva makumi matanhatu, uye morphology uye kuita kweiyo reagent kuramba isina kuchinjika.
Zvakajairika kupisa kuchengetedza uye kufambisa:Hapana chinodiwa cheni inotonhora, hapana chikonzero chekuchengeta pakudziya kwakadzika usati wasunungura, kusunungura zvachose nzvimbo inotonhora yekuchengetedza.
Yakagadzirira-yekushandisa:Lyotolizing yezvikamu zvese, hapana chikonzero chekugadziriswa system, kudzivisa kurasikirwa kwezvinhu nekwakakwirira viscosity senge enzyme.
Multiplex zvinangwa mune imwe tube:Iyo yekuona chinangwa chinofukidza Novel coronavirus orf1ab gene, n gene, s ger kuti vadzivise hutachiona. Kuti uderedze nhema dzenhema, renase pra inoshandiswa seyamari yemukati, kuitira kuti usangane nekiriniki inoda sampuli yemhando yekutonga kwemhando yepamusoro.
