Novel Coronavirus (SARS-CoV-2) Multiplex Chaiyo-Nguva PCR Kit
Iyi PCR kit inonzwisisika zvakanyanya, yakagadzirira kushandisa inowanikwa mu lyophilized fomati (kuomesa-kuomesa) kuchengetedza kwenguva refu.Iyo kiti inogona kutakurwa uye kuchengetwa mukamuri tembiricha uye inogadzikana kwegore rimwe.Imwe neimwe chubhu ye premix ine ese mareagents anodiwa pakukwidziridzwa kwePCR, kusanganisira Reverse-transcriptase, Taq polymerase, primers, probes, uye dNTPs substrates.Inongoda kuwedzera 13ul mvura yakasvibiswa uye 5ul yakabviswa RNA template, ipapo inogona kumhanya uye kukwidziridzwa paPCR zviridzwa.
Muchina weqPCR unofanirwa kuita zvinotevera zvinodiwa:
1. Fit 8 strip PCR tube volume 0.2 ml
2. Iva neanopfuura mana ekuona nzira:
| Channel | Kufadza (nm) | Emission (nm) | Pre-Calibrated Dyes |
| 1. | 470 | 525 | FAM, SYBR Green I |
| 2 | 523 | 564 | VIC, HEX, TET, JOE |
| 3. | 571 | 621 | ROX, TEXAS-RED |
| 4 | 630 | 670 | CY5 |
PCR-mapuratifomu:
7500Real-Time PCR System, Biorad CF96, iCycler iQ™ Real-Time PCR Detection System, Stratagene Mx3000P, Mx3005P
Kuoma kwekutonhora kwekutakura cheni yeNovel Coronavirus nucleic acid yekuona reagent
Kana yakajairwa nucleic acid yekuona reagents inotakurwa kure kure, iyo (-20 ± 5) ℃ inotonhora cheni yekuchengetedza uye yekufambisa inodiwa kuti ive nechokwadi chekuti bioactive ye enzyme mune reagents inoramba ichishanda.Kuti uve nechokwadi chekuti tembiricha inosvika pachiyero, makirogiramu akati wandei echando chakaoma anodiwa pabhokisi rega rega reiyo nucleic acid yekuongorora reagent kunyange isingasviki 50g, asi inogona kungogara kwemazuva maviri kana matatu.Pamaonero ekuita kweindasitiri, huremu chaihwo hwemareagents akapihwa nevagadziri huri pasi pegumi muzana (kana kure kure kupfuura kukosha uku) kwemudziyo.Huremu huzhinji hunobva muchando chakaoma, ice packs uye furo mabhokisi, saka mutengo wekufambisa wakakwira zvakanyanya.
Muna Kurume 2020, COVID-19 yakatanga kubuda pamwero mukuru kunze kwenyika, uye kudiwa kweNovel Coronavirus nucleic acid yekuona reagent kwakawedzera zvakanyanya.Kunyangwe nemutengo wakakwira wekutengesa kunze ma reagents mucheni inotonhora, vazhinji vanogadzira vanogona vachiri kuigamuchira nekuda kwehuwandu hukuru uye purofiti yakakura.
Nekudaro, nekuvandudzwa kwemitemo yenyika kunze kwenyika yezvigadzirwa zvinorwisa denda, pamwe nekuvandudzwa kwekutonga kwenyika pamusoro pekuyerera kwevanhu uye zvinhu, kune kuwedzera uye kusava nechokwadi munguva yekufambisa yemareagents, izvo zvakakonzera matambudziko ane mukurumbira echigadzirwa akakonzerwa. nechokufambisa.Yakawedzerwa nguva yekufambisa (nguva yekufambisa ingangoita hafu yemwedzi yakajairika) inotungamira mukutadza kwechigadzirwa kazhinji kana chigadzirwa chasvika kune mutengi.Izvi zvanetsa mazhinji nucleic acid reagents ekunze mabhizinesi.
Lyophilized tekinoroji yePCR reagent yakabatsira kufambisa kweNovel Coronavirus nucleic acid yekuona reagent pasi rese.
Iyo lyophilized PCR reagents inogona kutakurwa uye kuchengetwa mukamuri tembiricha, izvo zvisingagone chete kuderedza mutengo wekufambisa, asi zvakare kudzivirira matambudziko emhando anokonzerwa nemaitiro ekufambisa.Naizvozvo, lyophilizing iyo reagent ndiyo nzira yakanakisa yekugadzirisa dambudziko rekutakura kunze kwenyika.
Lyophilization inosanganisira kuomesa mhinduro mune yakasimba mamiriro, uyezve sublimate uye kuparadzanisa mhute yemvura pasi pevacuum mamiriro.Iyo yakaomeswa solute inoramba iri mumudziyo ine yakafanana kuumbwa uye basa.Kuenzaniswa neyakajairwa mvura reagents, iyo yakazara-chikamu lyophilized Novel Coronavirus nucleic acid yekuona reagent inogadzirwa neLiming Bio ine zvinotevera maitiro:
Kugadzikana kwekupisa kwakanyanya:inogona nekumira kurapwa pa56 ℃ kwemazuva makumi matanhatu, uye morphology uye kushanda kweiyo reagent inoramba isingachinji.
Normal tembiricha kuchengetedza uye chekufambisa:hapana chikonzero chekutonhora cheni, hapana chikonzero chekuchengeta pane yakaderera tembiricha usati wasununguka, sunungura zvizere nzvimbo yekuchengetera inotonhora.
Yakagadzirira-kushandisa:lyophilizing yezvikamu zvose, hapana chikonzero chekugadzirisa hurongwa, kudzivisa kurasikirwa kwezvikamu zvine high viscosity yakadai se enzyme.
Multiplex tarisiro mune imwe chubhu:iyo yekuona tarisiro inovhara novel coronavirus ORF1ab gene, N gene, S gene kudzivirira hutachiona genovariation.Kuti uderedze nhema dzisina kunaka, RNase P gene yemunhu inoshandiswa sekutonga kwemukati, kuitira kuti isangane nekiriniki yekuda kwemuenzaniso wekutonga kwemhando.
