Yakasimba system mudziyo weSers-Cov-2 Antigen Rapid Test
Yakatarisirwa kushandiswa
Yakasimba System System yeSyar-COV-COV-2 Antigen Rapid Fours Inoshandisa Imunographato Iyi bvunzo ndeye imwe shandisa chete uye yakagadzirirwa sei-kuyedza. Zvinokurudzirwa kushandisa bvunzo iyi mukati memazuva manomwe ezviratidzo zvekutsigirwa zvinotsigirwa nekiriniki yekuongorora.
Nhanganyaya
Iyo novel coronaviruse ndeyo 0 genus. Chand-19 idanho rekufema rinorema rinotakura chirwere chinotapukira. Vanhu vanowanzo nyorwa. Parizvino, varwere vakatapukirwa neNovel Coronavirus ndidzo huru yehutachiona hwehutachiona hwehutachiona hwenzvimbo, nguva yekutsvaira ndeyekuti mazuva gumi nemana, kazhinji mazuva manomwe kusvika manomwe. Kuratidzwa kukuru kunosanganisira fivha, kuneta uye kuoma kukosora. NASAL Conesttion, mhino yemhino, maronda ehuro, Myalgia uye Diarrhea inowanikwa mune mashoma mashoma.
Nheyo
Iyo yakasimba ® System mudziyo weSars-COV-2 Antigen Test Account Kana iyo sampu ine sosi-cov-2 n protein antigun.it inoonekwa uye yakasungwa neAntiboys yakanyorwa pane iyo Latex nzvimbo yekuumba. Kana iyo yakaumbwa immune yakaoma kutama kune nitric acid fibrane membizi yekuona mutsara, kuti ufadze fuchsia yekuona mutsara (T-lins), kuratidza mafuro-cov-2 antigen yakanaka; Kana iyo t-mutsara usingaratidze ruvara, mhedzisiro yakaipa. Mumwe mutsetse pane nitric acid fiber memrane yakarongedzwa neiyo strepavididin antibodies semhando yekudzora mhando (c mutsara), kuratidza maitiro ekuyedza.
ZVEKUNGWARIRIRA
• Iyi kit ndeye mu vitro diagantics chete.
• Iyi kit inogona kutungamirwa nevashandi vekurapa kana vasiri vekurapa nekutevera mirairo yekushandisa.
• Verenga mirairo inyatsonatsa usati waita bvunzo.
• Ichi chigadzirwa hachina chero chinhu chinowanikwa nevanhu.
• Usashandise kit Mukati mushure mekupera zuva.
• Bata zvese zviyero sezvinogona kutapukira.
• Usaite pipette reagent nemuromo uye hapana kuputa kana kudya uchiita fungidziro.
• Pfeka magurovhosi panguva yese iyi.
Kuchengetera uye Kugadzikana
Mafuro akasungwa muedzo kit inogona kuchengetwa pakati pe2-30 ° C yenguva yehupenyu hwesherufu nekuti yakaratidza muhomwe muhomwe.
Kuunganidza kuunganidza uye kuchengetedza
Best Saliva Fortimen inofanira kuunganidza mangwanani mushure mekunge imbomira. Usadye kana kunwa chero chinhu kwemaminitsi makumi matatu usati waunganidza sosi yako sampu. Zviite usati uine kofi, kudya kwemangwanani, kana kutsvaira mazino ako - kana kumirira kusvika usati wapedza chero chinhu mumaminitsi makumi matatu apfuura.
Maitiro
Huya nemiedzo yekumisa tembiricha (15-30 ° C) usati washandiswa.
Nhanho 1:
Vhura bhegi, tora mudziyo wekuedza, vhura chifukidzo chechigumo chebvunzo yebvunzo.
Nhanho yechipiri:
• Bata iyo lest cassette, isa iyo salsa adsorption danda pasi porurimi, ita kuti adsorption tsvimbo uye rurimi runokwana zvakanyatsoitika kwemasekondi zana nemashanu.
• Chengetedza mudziyo wakasarudzika uye kurega mvura yemvura kuti uende kumusoro kusvika kusvika patsva pamusoro peC, wozoburitsa cap yadzoka.
• Isa mudziyo wakakomberedzwa pane workbench.
Nhanho 3:
Re-nguva uye kuverenga iyo yekuona resih maminitsi gumi nemashanu gare gare.
Kurasa zvakaringana marara kuita Biihazi-ard mudziyo.
Mhando yekudzora
Yemukati maitiro ekutonga inosanganisirwa muedzo. Bhendi rebhuruu rinobuda munzvimbo yekutonga (c) inoonekwa seyomukati kuronga. Inosimbisa kukwana kwakakwana vhoriyamu uye kururamisa maitiro maitiro.
Zvisingakwanisi muedzo
1. Iyo kit inoitirwa kushandisa iyo yakakodzera kuwanikwa kweanopinda-cov-2 Atigens kubva pamasita.
2. Iyi bvunzo inoona zvese zvinoshanda (rarama) uye isiri-inobatsira-cov-2. Kuedza kuita kunoenderana nehuwandu hwehutachiona (antigen) mumuenzaniso uye zvinogona kana kuti zvinogona kunge zvisina kukodzera nemhedzisiro yemuenzaniso chakaitwa pane imwe chete sampuli.
3. Mhedzisiro isina kunaka inoitika kana iyo nhanho yeantigen mumuenzaniso iri pazasi pekuonekwa kwebvunzo kana kuti muenzaniso wakaunganidzwa kana kutakurwa zvisiri izvo.
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5. Iyo kit ndeye yekuzvidzivirira chete. Mhedzisiro dzisina kunaka hadziitonge kunze-ka-2 utachiona uye munhu asiri kutapukira. Kana zviratidzo zviripo, tsvaga zvakare kuedzazve kuongororwa.
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7. Mhedzisiro yebvunzo
8. Mhedzisiro isina kunaka yemibvunzo haina kuitirwa kutonga mune vamwe vasiri-scar hutachiona kana hutachiona hutachiona.
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10 Vashandisi vanofanirwa kuongorora kufungidzira nekukurumidza sezvinobvira mushure mekufungidzira kuunganidzwa.
11. Iko kunzwira tsitsi kwert-pcr assay mune kuongororwa kweCovid-19 inongova 50% nekuda kwekutsvagisa chikamu chekudzokorora, kuderera nekuda kwenzira yayo.
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Mhedzisiro yebvunzo
13. Monoclonal Antibodies anogona kukundikana kuona, kana kuona nekunyanyisa kunzwa, vanochengetedza mavairasi 2 ivhi vane madiki ane pasi pechidiki amino acid mune iyo tarisiro yenyika.
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15. Kuwanda kweanti imagine mumuenzaniso kunogona kuderera sezvo nguva yechirwere inowedzera.
Mifananidzo yakaunganidzwa mushure mezuva rechinomwe zvehurwere zvinokwanisika kuve zvisina kunaka zvichienzaniswa nert-pcr assay.
Kunzwa kwebvunzo mushure memazuva manomwe ekutanga kwezviratidzo zvave kuzivikanwa kuti kuderedza sekufananidzwa kwert-pcr asta.
16. Hazvikurudzire kushandisa hutachiona hwehutachiona hwehukama
17. Kugara uchiedzwa kunokosha kuwedzera kunzwa kwekuongororwa kwekuongororwa kwehuwandu-19.
18 B.1.351; B.1.2; B.1.1.28; B.1.617; B.1.1.529.
19. Mhedzisiro yakanaka inoratidza kuti vhezheni yemitambo yakaonekwa mumuenzaniso yakatorwa, ndokumbirawo kuzvidzora uye kuzivisa yako yemhuri kuchiremba uye / kana yako yehutano yedhipatimendi zvinoenderana nezvinodiwa neHurumende.
Yakanakisa chikamu chibvumirano: (PPA) = 98.02% (93.03% ~ 99.76%) *
Yakashata muzana chibvumirano: (NPA) = 100% (98.23% ~ 100%) *
Yakazara coincience rate = 98.76%
* 95% chivimbo chekupindirana
Kuongorora kwekuongorora
a) muganho wekuonekwa (Lood):
Iyo muganho wekuonekwa (Lod) weyedzo wacho wakatsanangurwa uchishandisa kudzikisira Dilutifiuti dzisina kujairika sers-cov-2. Kugadzirira kweanopfuurira-kwakanangana necoronavirus-2 (anofukunura-cov-2), anozvitsaura muChina CDC, iyo yakavezwa ne β -Prollactone. Izvo zvinyorwa zvakapihwa chando pakubata kwekusangana kweTcid50of 5.00 x105/ ml.
Kuti uone kuti anochengetedza-cov-2 kuratidza learsay kana achishandisa zvakananga mate. Muchidzidzo ichi anenge 50μl yehutachiona hwehutachiona hwakatsemurwa nemunguva yakaipa.
Iyo Lod yakanga yakatemwa mumatanho matatu:
1. Lod kuongorora
10-Fold Dilutions yeViriyo vasiri vehutachiona vakagadzirwa mune zvisina kunaka mate uye vakagadziriswa nekuda kwekudzidza kwega kwega sekutsanangurwa pamusoro apa. Aya dilutions akaedzwa mudhara. Iyo yekunyora inoratidza 3 ye3 positives yakasarudzwa kuti LOD range kutsvaga.
2. Lod range kutsvaga
Shanu (5) Dunhu Dunhu rakagadzirwa neTcid50of 5.00 x102/ ml kusangana mune zvisina kunaka saliva yakagadziriswa yekudzidza sekutsanangurwa pamusoro apa. Aya dilutions akaedzwa mudhara. Iko kusimbirira kuratidzira 3 ye3 positives yakasarudzwa kuti LOD kusimbiswa.
3. Lod kusimbiswa
Kusungwa kwetsic50of 2.50 x102/ ml dilution yakaedzwa kwehuwandu hwemakumi maviri (makumi maviri) mhedzisiro. Angangoita gumi nemapfumbamwe (19) of makumi maviri (20) mhedzisiro yaive yakanaka.
Mhedziso:
Zvichienderana nekuedza uku kusimbiswa kwasimbiswa se:
Lod: Tcid502.50 x102/ ml
b) kuyambuka-kuita:
Yambidzika System mudziyo unochengetedza-cov-2 antigen nekukurumidza bvunzo.
Ongororo imwe neimwe nehutachiona dzakaedzwa mumatare. Zvichienderana nedata inogadzirwa neiyi chidzidzo, iyo yakasimba ® mudziyo weSers-COV-2 Antigen nekukurumidza bvunzo hayambuke-ita zvisikwa kana mavhairasi akaedzwa.
c) kukanganisa chinhu:
Zvingangoita zvinhu zvekupindirana zveSimba reSimba Chimwe chinhu chimwe nechimwe chakaedzwa mune tsaona. Zvichienderana nedata inogadzirwa neiyi chidzidzo, iyo yakasimba® inochengetedza-cov-2 antigen nekukurumidza bvunzo hazvipindire zvinhu zvakaedzwa.
d) Hook mhedzisiro
Iyo yepamusoro yepamusoro yekupisa-isina mactivated sars-cov-2 stock inowanikwa (Tcid50of 5.00 x 105/ ml) yakaedzwa. Pakanga pasina hoko rakawanikwa.
